Gilson 402 User Manual

402 Dilutor DispenserUser’s Guide

Safety-2SafetyThis instrument1 must not be sterilized, using anautoclave, or any other mechanical device. Whenyou need to clean an instrument, use one

7-4Troubleshooting 7Electrical ProblemsElectrical ProblemsProblem Possible Cause SolutionInstrument does not Power cord not connected. Check power cor

Appendix A-1Parts & Accessories Lists Appendix AParts lists for the 402 Dilutor Dispenser, consisting of Standard Equipment,Additional Accessorie

Appendix A-2Parts & Accessories Lists Appendix A402 Single Syringe402 Single SyringePart Number Qty DescriptionF410514 1 402 Dilutor Dispenser (Si

Appendix A-3Parts & Accessories Lists Appendix A402 Dual Syringe with Tee Junction Configuration402 Dual Syringe with Tee JunctionConfigurationPar

Appendix A-4Parts & Accessories Lists Appendix AAdditional AccessoriesAdditional AccessoriesPart Number DescriptionF4015015 Adapter kit for dispos

Appendix A-5Parts & Accessories Lists Appendix ASpare PartsSpare PartsPart Number DescriptionF4015072 100 µL piston kitF4015073 250 µL piston kitF

Appendix A-6Parts & Accessories Lists Appendix A

Appendix B-1Chemical Compatibility Appendix BThis chapter contains information about the chemical compatibility of the syringesand the tubing.

Appendix B-2Chemical Compatibility Appendix BChemical CompatibilityChemical CompatibilityMaterials in Contact with LiquidsIn the 402 Dilutor Dispenser

Appendix C-1Technical Data Appendix CThis chapter contains the Technical Data concerning the 402 Dilutor Dispenser.

1-1Introduction 1The following chapter describes the instrument, the service and warranty conditions.

Appendix C-2Technical Data Appendix CHydraulicsHydraulicsDilution RatioWhen using the dual syringe model equipped witha 25 mL syringe on the left and

Appendix C-3Technical Data Appendix CHydraulicsSyringesPerformance SpecificationsThe following values are obtained using a definedprotocol. If this pr

Appendix C-4Technical Data Appendix CHydraulicsTypical values (all syringes)Syringe capacity Max. accuracy Max.error imprecision%+ A % RSD %100 0.50

Appendix C-5Technical Data Appendix CMechanicsMechanicsValveType: Three port, two position.Angle between ports: 120°.Valve drive: Stepper motor.Rotati

Appendix C-6Technical Data Appendix CEnvironmentalEnvironmentalConformity to NormsRefer to separate leaflet LT801354 ‘Declaration ofConformity’.Storag

Appendix D-1Error Messages Appendix DThis chapter contains the Error Messages concerning the 402 Dilutor Dispenser.

Appendix D-2Error Messages Appendix DError MessagesError Message Cause/Possible ActionAdjust volume too small In DISPENSE, you have, you have entered

Appendix D-3Error Messages Appendix DError MessagesError Message Cause/Possible ActionParameters incompatible! Refer to page 5-16.Ratio impossible Dil

Appendix D-4Error Messages Appendix D

Index-1Index 0Abort 4-10, 4-11Accessories list 4-5Acetonitrile B-2Air bubble free filling 4-10Air Gap 4-4, 4-12, 5-2, 5-6, 5-17Alcohol B-2Alternative

1-2Introduction 1Notational ConventionsNotational ConventionsThe conventions in this User’s Guide are as follows.Used in the stand-alone mode, this in

Index-2IndexInstallation 3-1Keypad 2-2, 3-8Large Volumes 5-4Liquid contact materials B-2Link 5-27Lock 5-11MaintenanceAutoclaving 6-5Changing the pisto

Index-3IndexTitrate 5-14Troubleshootingelectrical problems 7-4hydraulic problems 7-3mechanical problems 7-2Tubingconnecting 3-4transfer 3-4valve inlet

Index-4Index

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1-3Introduction 1OverviewOverviewThe 402 Dilutor-Dispenser, is used to transfer liquidsbetween vials according to user defined protocols(Methods). You

1-4Introduction 1Good Laboratory Practice (GLP) is facilitated by thefollowing:씮 codes to protect the program files against unau-thorized modification

1-5Introduction 1UnpackingUnpackingYour 402 Dilutor-Dispenser is shipped in a boxtogether with its various accessories. The originalbox and packing ma

1-6Introduction 1WarrantyIf the 402 Dilutor-Dispenser does not appear tofunction correctly, first verify the electrical connec-tions are correct and t

1-7Introduction 1Customer ServiceGilson and its worldwide network of authorizeddistributors provide you with four types of assist-ance: sales, technic

1-8Introduction 1

2-1Description 2The following chapter describes the mechanics and hydraulics of the Dilutor-Dispenser.Two models of 402 Dilutor-Dispenser are availabl

2-2Description 2Single-syringe ModelSingle-syringe ModelThe Single-syringe model has a Valve module at theleft, and a blanking plate at the right.The

2-3Description 2Dual-syringe ModelDual-syringe ModelThe Dual-syringe model is fitted with a Valvemodule at the left and a Tee-junction module at theri

2-4Description 2Keypad and ProbeKeypad and ProbeKeypadThe keypad includes an LCD display (two 24-character lines), membrane touch-keys, and aconnectin

2-5Description 2Hydraulic ComponentsValveThe two-position valve, which is fitted to the left-hand side of the 402, has three ports. It switchesthe syr

2-6Description 2Hydraulic ComponentsThere are seven syringe sizes available for the 402,all except the 25 mL are autoclavable. The lifetimeof all pist

3-1Installation 3This chapter explains how to install the fuses, syringes and hydraulics according toyour system requirements.This chapter explains ho

3-2Installation 3Assembling the ProbeFor Standard UseIn standard use the probe is used with the 2 mLtapered transfer-tubing (see opposite figure).1. T

3-3Installation 32 Special adapters for the probe1 D200 tips, pack of 101 D1000 tips, pack of 101 Transfer-tubing (outlet) non-tapered, FEP (2 mL)1. L

3-4Installation 3Connecting the TubingConnecting the TubingTubing connectors should be hand-tightened,tools should not be used as their use will damag

3-5Installation 3Connecting the TubingAfter assembling the probe, the other end of thetubing is installed by screwing the connector intothe right-hand

402 Dilutor DispenserUser’s GuideLT801310/C - ©2003 Gilson SAS All rights reserved November 2003

3-6Installation 3The transfer tubing connects the outlet port (right-hand) of the Tee-junction to the probe. A 2 mLtapered transfer-tubing is supplied

3-7Installation 3Electrical ConnectionsElectrical ConnectionsThe rear panel of the 402 Dilutor-Dispenser houses:- sector block containing the power so

3-8Installation 3Electrical ConnectionsConnecting the Power CordMake sure that the ON/OFF switch is in the OFF(down) position. Connect the power cord

3-9Installation 3SyringesSyringesThe 402 Dilutor-Dispenser is supplied withoutsyringes. Order a syringe (or syringes), appropriateto your Method, for

3-10Installation 3Upgrading to Dual-syringe ModelUpgrading to Dual-syringe ModelThe single syringe 402 can be upgraded to improvethe versatility of th

3-11Installation 3Upgrading to Dual-syringe ModelRemoving the Cover1) Undo the four screws (with two washers) at thebase of the 402 that secure the to

3-12Installation 3Inserting the ModuleDo not try to force the module into position as thismay affect the alignment of the component parts.You should p

4-1Operation - Standard Use 4This chapter describes how to operate the 402 in standard use. Chapter 5 containsmore detailed and advanced information,

4-2Operation - Standard Use 4Essential StepsEssential StepsHere is a summary of the things you must do, andthe order in which you should do them.1) Ch

4-3Operation - Standard Use 4Essential StepsIf you have a Dual-syringe 402, install the tubingsas described in Chapter 3, page 3-5. You mustconnect 4

4-4Operation - Standard Use 4Essential StepsConfiguration (Conf)You only have to enter the Configuration detailsone-time; the 402 keeps this informati

4-5Operation - Standard Use 4Essential StepsIf you want to change the configured volume, pressChge (until you reach the correct value); when thevolume

4-6Operation - Standard Use 4Essential StepsNext is used to go to the next screen (see Air FlowScreen),Prev is used to go to the previous screen (see

4-7Operation - Standard Use 4Essential StepsFit the Syringe(s)1) First you must lower the piston rod(s), as follows:- press the Conf key to access the

4-8Operation - Standard Use 4In-run PromptsYou can defer some parameters (volumes and flowrates) by entering a question mark (?) in place of thevalue.

4-9Operation - Standard Use 4For the Dispense task, press Disp; you see the firstparameter screen, Dispense Screen (1).Press Chge until you see ‘Sourc

4-10Operation - Standard Use 4Prime the 402 (Man)Prime is used to thoroughly clean out all fluidpathways with fresh solvent and to clear bubblesfrom t

4-11Operation - Standard Use 4‘Homing ...’ appears on the screen until the syringe(s)have returned to their uppermost (home) positions,during which ti

4-12Operation - Standard Use 4Essential StepsIn Run Screen (1)1air gap2liquid3fraction14fraction 25fraction 36fraction 47to wasteExtra VolumeIn Run Sc

4-13Operation - Standard Use 4(ii)Press the commandbutton to aspirate AIR.The command light issteady and you seealternately In RunScreens 3a and 3b.(i

Table of Contents-1Table of Contents 0Safety1 IntroductionNotational Conventions ... 1-

4-14Operation - Standard Use 4Press ‘Abort’ to confirm that you wish to stop andyou are returned to the Run Screen. Alternatively,you can bring the 40

5-1Operation - Advanced Use 5This chapter explains in details how liquids are aspirated and dispensed using the 402Dilutor-Dispenser and gives in dept

5-2Operation - Advanced Use 5Correct Use of the 402Correct Use of a 402Single-syringe Model• Samples and reagents are aspirated from theprobe side.• L

5-3Operation - Advanced Use 5Correct Use of the 402• If a drop of liquid is present at the end of thetubing after dispensing, you must include thisdro

5-4Operation - Advanced Use 5Correct Use of the 402Aspirating Large Volumes via theProbe1. The valve switches to connect the left-hand syringe to the

5-5Operation - Advanced Use 5SoftwareSoftwareYou must first enter the configuration details foryour 402 Dilutor-Dispenser. Then you may do oneof three

5-6Operation - Advanced Use 5Software4 Press the Next soft-key to go to the followingscreen.5 Press the Chge soft-key to choose an option or toselect

5-7Operation - Advanced Use 5SoftwareIf you want to change the configured volume, pressChge (until you reach the correct value); when thevolumes are t

5-8Operation - Advanced Use 5When you specify left and right syringe capacities(left must be > right), you must enter compatiblevolumes. An error m

5-9Operation - Advanced Use 5CountersUnder normal use you should expect to fit new (orrefurbished) syringes after 30000 cycles with water.This feature

Table of Contents-2Upgrading to Dual-Syringe Model ... 3-10Removing the Cover ...

5-10Operation - Advanced Use 5to access the required task and its parameters,(ii) if you have entered a syringe correction factor inthe configuration

5-11Operation - Advanced Use 5When you select Yes, the file is deleted from thedirectory and you see the New-File Directory Screen.Press ESC to return

5-12Operation - Advanced Use 5To unlock this file you must key in the code thatwas used to lock the file. You may not edit a lockedfile, so the Edit s

5-13Operation - Advanced Use 5Press Abort, when you want to stop priming;Initialization Screen 2 reappears. Ensure that theprobe-end of the transfer-t

5-14Operation - Advanced Use 5After completely aspirating the liquid being measured,you see (for example) the Measurement Screen.Procedure1) Aspirate

5-15Operation - Advanced Use 5This is realized using the 402 by pressing the probe’scommand button with increasing rapidity as youapproach the neutral

5-16Operation - Advanced Use 5KeysThe use of the soft-keys is as follows:- Prev is used to go to the previous task (or screenwithin a task),- Next is

5-17Operation - Advanced Use 5Chaining TasksBecause of the flexibility of the software manycombinations of the tasks are possible in your Method.Howev

5-18Operation - Advanced Use 5DiluteThree types of dilution task are available ‘Normal’,‘Unknown’, and ‘Serial’. Three modes are availablefor ‘Normal’

5-19Operation - Advanced Use 5Press Next, then key in the sample volume, pressENTER.Depending on the mode,you see one of threescreens after pressingNe

Table of Contents-3Changing the Piston Syringes and Piston Noses ... 6-7Changing the 100 and 250 µL Piston Noses ...

5-20Operation - Advanced Use 5For each dilution in the series:Vt = Vs x Rd, Vd = Vt - VsFor example, after selecting Dilution Ratio Mode, youenter Sam

5-21Operation - Advanced Use 5Optionally, for anymode, you can displaythe variables calculatedby the software bypressing Data.Optionally, you can modi

5-22Operation - Advanced Use 5Optionally, you can supply up to 12 reagents, pressReag, key in number of reagents, press ENTER.Press Next, then for eac

5-23Operation - Advanced Use 5If for any reason you want to repeat the measure-ment (e.g. you need to select the LEFT syringeinstead of the RIGHT), pu

5-24Operation - Advanced Use 5volume of liquid, is dispensed, at the end of theprocess, into the waste.When you configure disposable tips in air mode,

5-25Operation - Advanced Use 5Press ESC (twice).Press ESC.PipetteYou use this task to aspirate a series of fractions viathe probe, then dispense the t

5-26Operation - Advanced Use 5Optionally at the first of the volume screens, pressFlow.Key in a value for Aspflow, press ENTER.Press Next.Key in a val

5-27Operation - Advanced Use 5Press Mix, then select the mixing mode (air or asp/disp).Press Chge to switchmodes.Press ENTER, thenNext.Key in volume,

5-28Operation - Advanced Use 5Key in the file number of the second Method tolink(8), press ENTER, press Next.Key in the file number of the third Metho

5-29Operation - Advanced Use 5Value for use withFile Number TASK/Parameter Dual-syringe Model Single-syringe ModelMethod 92 DispenseSample Source Res

5-30Operation - Advanced Use 5When you have finished, or wish to change programs,press STOP, to instantly halt the program. Alterna-tively, you can br

5-31Operation - Advanced Use 5Additional InformationFlow RatesFlow rates depend on the nature of the liquid(viscosity), the capacity of the syringe, a

5-32Operation - Advanced Use 5Additional InformationFlow Rates - Dual-syringe ModelFlow rates are the values entered in Flow menus(Asp Flow or Disp Fl

5-33Operation - Advanced Use 5Syringe Correction FunctionA correction factor may be applied to all volumes ofliquid to compensate for the effects of t

5-34Operation - Advanced Use 5Gilson D200 tips are used for volumes from 1 µL to78 µL (with a 100 µL syringe, minimum recommendedair-push-volume is 20

6-1Maintenance 6This chapter explains how to perform the routine maintenance procedures.The only maintenance operations necessary for the 402 DD are:•

6-2Maintenance 6CleaningCleaningCleaning the ExteriorThe instrument cover and front panel may be cleaned.Ensure that the instrument is switched OFF an

6-3Maintenance 6Cleaning1) Pull the axle away from the valve main body.2) Remove the ceramic stator from the valve axle.3) Tap the valve inner body a

6-4Maintenance 6CleaningCleaning the Syringe AssemblyYou must dismount the syringe and piston assemblyfrom the instrument and then separate the syring

6-5Maintenance 6AutoclavingAutoclavingCertain parts of the 402 can be autoclaved (20 min.at 0.1 MPa, 121° C). They must be removed fromthe instrument

Safety-1Safety 0Read this section carefully before installing and operating the instrument.For safe and correct use of the instrument, it is essential

6-6Maintenance 6Chaning the Syringe Piston AssemblyChanging the Syringe PistonAssemblyFor all syringe sizes, it is necessary to replace thepiston asse

6-7Maintenance 6Changing the Piston Syringes and Piston NosesChanging the Piston Syringes andPiston NosesThe piston nose should be replaced after the

6-8Maintenance 61. Remove the syringe from the pump as describedin Chapter 4.2. Slide the piston out of the syringe barrel.3. Remove the piston nose f

6-9Maintenance 6Changing the Syringe Piston and Piston NosesChanging the 500 µL Piston Noses1. Remove the syringe from the pump as describedpage 6-4.2

6-10Maintenance 6Changing the Piston Syringes and Piston NosesBefore Mounting a 500 µL Syringe: To optimizethe fitting of the 500 µL syringe on the Di

6-11Maintenance 6Changing the Syringe Piston and Piston NosesChanging the 25 mL Piston NosesYou don’t need a special tool for mounting thepiston nose

6-12Maintenance 6Changing the Piston Syringes and Piston NosesPiston shaftPistonnosePiston shaft holderPiston nose holderO-ring5. Insert the new pisto

7-1Troubleshooting 7This chapter explains how to diagnose possible problems and how to overcome them.

7-2Troubleshooting 7Mechanical ProblemsMechanical ProblemsProblem Possible Cause SolutionValve does not fit on to the 402. Valve axle not properly al

7-3Troubleshooting 7Hydraulic ProblemsHydraulic ProblemsProblem Possible Cause SolutionLiquid leakage Connections incorrectly made. Tighten all tubing